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The Luciferase Reporter Assay: How it works 26 Aug 2013 | 04:59 pm
The luciferase reporter assay is commonly used as a tool to study gene expression at the transcriptional level. It is widely used because it is convenient, relatively inexpensive, and gives quantitati...
Where to publish negative results 21 Aug 2013 | 12:30 pm
I met a final year PhD student once, who told me a sad story. His supervisor had a plausible idea that exercise reduces the chances of developing bowel cancer. To test the hypothesis, the student made...
8 more helpings of free PC Software for biological scientists 19 Aug 2013 | 04:15 pm
It’s been while since we talked about free PC software for biologists. So we thought we’d treat you to 8 more ideas that can turn you PC in to a super-bio-research-enabling-machine….. 1. Silvernote St...
Breaking Up is (Not That) Hard to Do: Sonication for Cell Lysis 19 Aug 2013 | 11:00 am
To answer some of the more interesting research questions, you often need to get a good look at what’s going on inside the cell. Whether you’re running a Western blot or measuring enzyme activity, man...
An Easy Way to Start Using R in Your Research: Basic Tutorial 14 Aug 2013 | 11:00 am
So now you’re convinced that R is the language for you, you’ve downloaded R-Studio (from http://www.rstudio.com/) and opened it, and. . .what the hell do you do now? Great question! I always find it ...
Why you shouldn’t be slow in your response to prospective employers in science 12 Aug 2013 | 04:48 pm
We live in an age if instant gratification. Instant streaming, instant Facebook updates, instant Tweets. When do I want it? Right now, of course. In this age of “instantness”, it is crucial as the job...
How to properly analyze and troubleshoot DNA sequencing results 7 Aug 2013 | 07:18 pm
As part of my job ensuring plasmid quality at Addgene, I analyze 50-100 sequencing reactions a week. So I have developed some good habits that I wanted to pass on to you to make sure you are getting t...
Book review: D.T. Max: The family that couldn’t sleep: a medical mystery 5 Aug 2013 | 12:30 pm
Imagine: you are an average middle-aged relatively healthy person. One day you start sweating. When you look at yourself in the mirror you see that your pupils have shrunk to pinpricks. You have notic...
An Easy Way to Start Using R in Your Research – Introduction 31 Jul 2013 | 11:00 am
Working with large datasets can be very frustrating and time consuming. If only there were more tools out there to simplify things without needing to invest a PhD’s worth of time to learn how to use ...
Alternatives to presenting your science with Powerpoint 29 Jul 2013 | 05:59 pm
I was shocked recently at a seminar called “Writing with style” by the Manchester University writer-in-residence, Chris Simms. He opened by saying that he has never done a presentation using Powerpoi...
