Oxfordjournals - peds.oxfordjournals.org - Protein Engineering Design and Selection - current issue
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Rational engineering of the fungal P450 monooxygenase CYP5136A3 to improve its oxidizing activity toward polycyclic aromatic hydrocarbons 23 Aug 2013 | 07:04 pm
A promising polycyclic aromatic hydrocarbon-oxidizing P450 CYP5136A3 from Phanerochaete chrysosporium was rationally engineered to enhance its catalytic activity. The residues W129 and L324 found to b...
Structural investigation of influenza virus hemagglutinin membrane-anchoring peptide 23 Aug 2013 | 07:04 pm
Hemagglutinin (HA), the trimeric spike of influenza virus, catalyzes fusion of viral and cellular membranes. We have synthesized the anchoring peptide including the linker, transmembrane region and cy...
Peptidyl-prolyl isomerase activity of FK506 binding protein 12 prevents tau peptide from aggregating 23 Aug 2013 | 07:04 pm
The Alzheimer's disease-related protein, tau, aggregates into neurofibrillary tangles when it is hyperphosphorylated. The amino acid sequence included in the third repeat (R3) of the microtubule-bindi...
Efficiency of puromycin-based technologies mediated by release factors and a ribosome recycling factor 15 Jul 2013 | 07:04 pm
Two puromycin-based techniques, in vitro virus (IVV) and C-terminal labelling of proteins, were developed based on the observation that puromycin binds the C-terminus of a protein. Puromycin technolog...
Chaperone-like effects of a scFv antibody on the folding of human muscle creatine kinase 15 Jul 2013 | 07:04 pm
Molecular chaperones play an essential role in assisting the folding of a myriad of nascent peptides to form different biologically active proteins. Therefore, their low substrate specificity is impor...
Isolation of {alpha}L I domain mutants mediating firm cell adhesion using a novel flow-based sorting method 15 Jul 2013 | 07:04 pm
The inserted (I) domain of αLβ2 integrin (LFA-1) contains the entire binding site of the molecule. It mediates both rolling and firm adhesion of leukocytes at sites of inflammation depending on the ac...
Early steps in thermal unfolding of superoxide dismutase 1 are similar to the conformational changes associated with the ALS-associated A4V mutation 15 Jul 2013 | 07:04 pm
There are over 100 mutations in Cu/Zn superoxide dismutase (SOD1) that result in a subset of familial amyotrophic lateral sclerosis (fALS) cases. The hypothesis that dissociation of the dimer, misfold...
PASylation: a biological alternative to PEGylation for extending the plasma half-life of pharmaceutically active proteins 15 Jul 2013 | 07:04 pm
A major limitation of biopharmaceutical proteins is their fast clearance from circulation via kidney filtration, which strongly hampers efficacy both in animal studies and in human therapy. We have de...
Compartmentalization of destabilized enzyme-mRNA-ribosome complexes generated by ribosome display: a novel tool for the directed evolution of enzymes 24 Jun 2013 | 06:44 pm
We have developed an in vitro evolution method for the selection for catalytic activity under the conditions of free intermolecular interaction between the enzyme and a substrate. The destabilized ter...
Transmitting the allosteric signal in methylglyoxal synthase 24 Jun 2013 | 06:44 pm
The homohexameric enzyme methylglyoxal synthase (MGS) converts dihydroxyacetone phosphate (DHAP) to methylglyoxal and phosphate. This enzyme is allosterically inhibited by phosphate. The allosteric si...
