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SEQstandards and MINSEQE: Minimum Information about a high-throughput SeQuencing Expt 14 Nov 2012 | 01:20 am
Introduction to the forum: a new standards page at the SEQWiki. Our new Wiki page hopes to be a quick and easy source of standards guidance from FGED-MINSEQE as well as various other global standards...
Oxford Nanopore @ ASHG 7 Nov 2012 | 11:29 pm
New thread started for ONT discussion as the old one was getting long. Also, GridION spotted in the wild? So it's not a hollow box... A call out to all ASHG attendees to please document ONT's announ...
PostDoc(s) to join a Medical Epigenomics Lab in Vienna, Austria 19 Aug 2012 | 08:02 pm
We are looking for enthusiastic students and postdocs to *join the Medical Epigenomics Lab at the CeMM* Research Center for Molecular Medicine of the...
Experienced Bioinformatician and/or Postdoc in Computational Genomics, Vienna Austria 19 Aug 2012 | 07:56 pm
We are looking for an *experienced bioinformatician* and/or a *postdoc in computational genomics* to join the *new Biomedical Sequencing Facility at...
Wierd overlapping paired ends with bimodal paired end distance distance distribution 19 Aug 2012 | 11:02 am
Hi Group! I'm dealing with quite the strange sequencing run. We don't normally do PE, so we don't have a standard operating procedure to fall...
Basic Nextera question 18 Aug 2012 | 09:54 pm
This may be a silly question, but I've been struggling to find the answer. I'll be using the Nextera dual indexing kit to prepare a single sample...
Could there be no SNPs in a homozygous genome assembly? 18 Aug 2012 | 04:19 pm
Hi everyone, I am having a difficult time in dealing with the SNPs from a homozygous genome. Theoretically speaking, I suppose there should be no...
Poor quality bacterial RNA, need help! 18 Aug 2012 | 06:07 am
Dear Friends, I am stuck on step '0' on my bacterial RNAseq project, i.e. poor quality RNA. My Experion runs look like this (see attached file)....
Shearing plant chromatin using Covaris S220 18 Aug 2012 | 05:01 am
Does anyone have suggestions for *shearing chromatin* isolated from Arabidopsis leaf tissue (or any plant material, for that matter) using the...
Library automation with E220 and Sciclone 18 Aug 2012 | 02:45 am
We are considering Covaris E220 (either parallel or serial) and Caliper (PerkinElmer) Sciclone to improve NGS library prep automation. Any...
